Analysis flowchart

A total of 2485 entries of Sequence Read Archive (SRA) from Human small RNAseq samples containing trillion of bases of total sum size 1.23 TB were retrieved and processed using SRA Toolkit. Adapter trimming and sequence quality filtering was done using ea-utils. The resulting filtered fastq files are indexed using in-house developed algorithm. The index files were compiled using using […]

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NepSeq algorithm

With the advent of next generation sequencing in 2008, the volume of biological data is growing exponentially. The sequence data are retained by NCBI Sequence Read Archive (SRA) at National Institutes of Health’s (NIH), EBI Sequence Read Archive (ERA) at European Bioinformatics Institute (EBI) and DDBJ Sequence Read Archive (DRA) at DNA Data Bank of

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